Construction and characterization of an expressed sequenced tag library for the mosquito vector armigeres subalbatus george f mayhew1, lyric c bartholomay2, hangyen kou3, thomas a rocheleau 1, jeremy f fuchs1, matthew t aliota, iyu tsao3, chiungyen huang3, tzetze liu4, kwangjen hsiao5, shihfeng tsai4,6. Armigeres subalbatus is known to be the vector for parasites of many human diseases like malaria, japanese encephalitis, filariasis etc. Read molecular cloning, characterization and tissue expression of prophenoloxidase cdna from the mosquito armigeres subalbatus inoculated with dirofilaria immitis microfilariae, insect molecular biology on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. A total of 11,761 adults, comprising 19 species belonging to 6 genera were identified. Help pages, faqs, uniprotkb manual, documents, news archive and biocuration projects. Pdf departments of parasitology and zoology, university of. Citeseerx development of a comparative genetic linkage.
Effect of prophenoloxidase expression knockout on the. Distribution and phylogeny of wolbachia strains in wild. Seven species of mosquitoes oviposited in open and bored bamboo traps at a site in peradeniya, sri lanka, the most prevalent being aedes albopictus, aedes novalbopictus and armigeres subalbatus. Methods and results from june to september 2018, mosquito habitat. Page 9, the present susceptibility status of armigeres subalbatus to hydrochlorin and different argano phosphorus insecticides. Armigeres subalbatus is a natural vector of the filarial worm brugia pahangi, but it rapidly and proficiently kills brugia malayi microfilariae by melanotic encapsulation. I have just modified one external link on armigeres subalbatus. Armigeres subalbatus mosquitoes rapidly destroy invading b.
Entomopathogenic or insect pathogenic nematodes are of particular importance for the control of insect pests and vectors of pathogens, while insectborne nematodes cause serious diseases in humans. Evaluation of larvicidal activity of medicinal plant. Culex pipiens pallens n 1620, aedes albopictus 806, armigeres subalbatus 377, anopheles sinensis 168, and culex tritaeniorhynchus 80. Aedes aegypti 10 %, armigeres subalbatus 3 % and culex pseudovishnui 2 % were the main species. Effect of prophenoloxidase expression knockout on the melanization of microfilariae in the mosquito armigeres subalbatus. Host innate immunity plays a central role in detecting and eliminating microbial pathogenic infections in both vertebrate and invertebrate animals. Functions of armigeres subalbatus ctype lectins in innate. Description of the transcriptomes of immune responseactivated hemocytes from the mosquito vectors aedes aegypti and armigeres subalbatus. Albopictus was found to be the dominant and frequently distributed specie. Development of a comparative genetic linkage map for armigeres subalbatus using aedes aegypti rflp markers michael t. Predatorprey interactions and the cannibalism of larvae of.
Study on vector mosquito of zoonotic brugia malayi in musi. To our knowledge, this is the first report of an established armigeres mosquito cell line. Rhythms in the biting behaviour of a mosquito armigeres subalbatus. Of the clusters, 66% of those from aedes aegypti and 62% of those from armigeres subalbatus are composed of a single sequence singletons. Molecular cloning, characterization and tissue expression of. Armigeres obturbans, armigeres aureolineatus, moreh, jiribam, annual 1. Osa knocking down freeflight adult mosquitoes via dynamic. Most recently, it was shown that melanization andor phagocytosis is elicited by a variety of bacterial species, independent of the gram type 30. Armigeres subalbatus is a natural vector of the filarial worm brugia pahangi, but it kills b. Larval habitats of armigeres subalbatus were delineated in urban and rural areas of pondi cherry. In order to mine the transcriptome for new insight into the cascade of events that takes place in response to infection in this mosquito, 6 cdna libraries were generated from tissues of adult female mosquitoes.
Melanization is an effective defence reaction used by mosquito hosts to kill malarial and filarial worm parasites. Mosquito transcriptome profiles and filarial worm susceptibility in. Majority of aedes albopictus was encountered in july from larval habitat types. Construction and characterization of an expressed sequenced. Ijmrk2 cibarium of armigeres kuchingensis edwards with the. Wolbachia are a group of maternally inherited intracellular bacteria known to be widespread among arthropods.
This project has been funded in whole or in part with federal funds from the national institute of allergy and infectious diseases, national institutes of health, department of health and human services, under contract no. Immunocytochemical localization showed that prophenoloxidase propo was distributed in the wound site. Wolbachia infections were found only among four mosquito species in sri lanka. Phylogenetic analysis of the wsp sequences showed two major branches confirming identities obtained from the pcr screening with strainspecific primers. Here, we present results from a season long study where we studied spatiotemporal abundance patterns of ar. Japanese encephalitis virus isolation from fieldcaught mosquitoes. Population dynamics of mosquitoes in various breeding.
Jan 01, 2000 potential role of armigeres subalbatus diptera. Transmission electron microscopy identified icosahedral, nonenveloped virus particles with a mean. Culex quinquefasciatus 83 %, aedes aegypti 10 %, armigeres subalbatus 3 % and culex pseudovishnui 2 % were the main species. Our preliminary entomological survey at that time suggested the mosquito species armigeres subalbatus as the vector of the zoonotic infections. It is very difficult to distinguish both the species except sternites vvi being pale scaled in armigeres armigeres kuchingensis edwards.
Establishment and characterization of two new cell lines. Melanization in response to wounding is ancestral in. Jan 01, 2000 armigeres subalbatus coquillett was the most susceptible species, indicating its status as a potential vector. The virus particles were spherical, 70 nm in diameter, and enveloped. A double subgenomic sindbis virus was used to silence ddc and. The use of gene silencing to study the role of dopa. Background the mosquito, armigeres subalbatus, mounts a distinctively robust innate immune response when infected with the nematode brugia malayi, a causative agent of lymphatic filariasis. Abdul hakeem college, melvisharam 632 509, vellore district, tamil nadu, india contents lists available at. It is found in sri lanka, 1 india, bangladesh, myanmar, pakistan, nepal, japan, china, korea, taiwan, ryukyuretto, indochina, thailand, and guam.
This page was last edited on 15 january 2020, at 18. An armigeres subalbatus cdna encoding ddc was cloned and real. Larva carnivorous and can be found from pools with foul water or water with a. In the initial wounding, coagulation and wound plug formation occurred with granulocyte migration.
Citeseerx document details isaac councill, lee giles, pradeep teregowda. Survey of various potential mosquito breeding habitats. Empirically, the visiblenearinfrared absorption spectra of three mosquito species, namely, culex piplens molestus, aedes albopictus, and armigeres subalbatus, were acquired to examine the disparity in photoabsorption property among different mosquito species and help determine an optimal wavelength for injuring the insects. Investigation of mosquito larval habitats and insecticide. In this study, predatory feeding patterns and cannibalism of larvae of armigeres subalbatus mosquitoes were quantified under laboratory conditions using aedes albopictus and culex uniformis larvae as prey organisms. From july 1997 to june 1998, a total of 123 pools of fieldcaught ar. Nevertheless, its population dynamics have been relatively poorly studied. Identification and functional analysis of the peptidoglycan recognition protein ld gene in the mosquito, armigeres subalbatus. Bhk21 cells infected with yn12031 exhibited an evident cytopathic effect cpe 32 h postinfection.
In this present report, we provide biological evidence to confirm. A total of 11,952 aedes aegypti and 12,790 armigeres subalbatus sequences yielded 2,686 and 2,107 est clusters, respectively. Anopheles sinensis, armigeres subalbatus, china, mosquito, plasmodium relictum. It is found in sri lanka, india, bangladesh, myanmar, pakistan, nepal, japan, china, korea, taiwan, ryukyuretto, indochina, thailand, and guam. Hemocytemediated phagocytosis and melanization in the mosquito armigeres subalbatus following immune challenge by bacteria. Detection and shooting process of dynamincally tracking an armigeres subalbatus. Text is available under the creative commons attributionsharealike license. Pdf mosquito transcriptome changes and filarial worm. Previously, we identified five pos, designated aspropo iv, from the mosquito armigeres subalbatus and demonstrated that the functions of aspropo i, ii and iii, were associated with filarial parasite melanization, blood feeding and cuticle formation, respectively. In this study, we isolated a virus strain yn12031 from specimens of armigeres subalbatus collected in the chinalaos border. The ascertaining of the two species with the molecular work will the future perspective and exploration more species of the genus will have enriched the diversity of the state and india as whole. Armigeres armigeres subalbatus is a species complex of zoophilic mosquito belonging to the genus armigeres. Another possible factor is the presence of cibarial armature buccopharyngeal spicules or teeth lo cated at the posterior margin of the cibarium preoral food cavity. The incidence of abnormal spermatozoa in normal and dichlorvostreated armigeres subalbatus was examined morphologically by electron microscopy.
Evaluation of larvicidal activity of medicinal plant extracts against three mosquito vectors a bagavan, a abdul rahuman unit of nanotechnology and bioactive natural products, post graduate and research department of zoology, c. Identification of a newly isolated getah virus in the. Aedes albopictus, culex quinquefasciatus, armigeres subalbatus and mansonia uniformis. If you have any questions, or need the bot to ignore the links, or the page altogether, please visit this simple faq for additional information. Isolation and fulllength sequence analysis of armigeres. Evaluation of larvicidal activity of medicinal plant extracts. Identification of a newly isolated getah virus in the china. However, both the species can be distinguished on the basis of new taxonomic characters. Aedes albopictus in contrast showed a downward trend from january to march but after march it showed an upward trend. Over the past few decades, researchers were curious to use wolbachia as a biological tool to control mosquito vectors.
A rickettsia nestedpcr targeting the variable domain of glta showed rickettsia felis in four mosquito species of 16 provinces cx. Mosquito transcriptome changes and filarial worm resistance. Population dynamics of armigeres subalbatus diptera. Pdf departments of parasitology and zoology, university. These three species preferred to lay in open than in bored traps. Late instar larvae of armigeres subalbatus are cannibalistic.
Citeseerx mosquito transcriptome profiles and filarial. Development of a comparative genetic linkage map for. Previously, this species has never been considered a medically important mosquito in malaysia as compared to other mosquitoes such as culex, aedes, mansonia and anopheles spp. Christensen1 university of wisconsinmadison, department of animal health and biomedical sciences. One of the causative agents of lympahtic filariasis is the nematode parasite brugia malayi that requires a competent mosquito vector for its development and transmission. The predatory nature of armigeres subalbatus larvae was examined to determine the possible use of this mosquito species in controlling cooccurring vector mosquitoes. The dual roles of armigeres subalbatus prophenoloxidase v in. The mosquitos used in this study had been maintained in the laboratory for 160 generations after being collected in kanagawa prefecture. In this present report, we provide biological evidence to confirm our.
Mar 01, 2002 hemolymph coagulation began almost immediately after wounding in mosquito, armigeres subalbatus, coquillett larvae. Armigeres subalbatus is widely distributed in malaysia. Armigeres subalbatus is a nuisance biter and a putative vector of japanese encephalitis and dengue viruses. Armigeres subalbatus colonization of damaged pit latrines. A revision of the illustrated taxonomic keys to genera and species of mosquito larvae of korea diptera, culicidae by lee, kwan woo 1999 5th medical detachment, 168th medical battalion 18th medical command, u. We successfully established two cell lines from the neonate larvae of a. The biting cycle of armigeres subalbatus is distinctly crepuscular, exhibiting two peaks of activity, a smaller one at dawn and a larger one at dusk. Culicidae in the transmission of japanese encephalitis virus in the absence of rice culture on liuchiu islet, taiwan weijune chen department of public health and parasitology, college of medicine, chang gung university, kweisan, taoyuan 33332, taiwan. Predation and cannibalism can affect the coexistence of mosquito species or the assemblage of mosquito species i. Full genome sequence analysis showed the virus to be related to members of the family totiviridae, and was therefore named armigeres subalbatus totivirus astv. Armigeres subalbatus incriminated as a vector of zoonotic. We studied its2 molecular characterization of two allopatric populations of this species. Ijmrk2 armigeres species of manipur, the easternmost. Files are available under licenses specified on their description page.
In the present study, we delineate the dual functions of aspropo v. Armigeres subalbatus, vector, zoonotic, brugia pahangi, filariasis. Pdf armigeres subalbatus incriminated as a vector of. During an investigation of arboviruses in china, a novel dsrna virus was isolated from adult female armigeres subalbatus. A novel lectin with a fibrinogenlike domain and its potential involvement in the innate immune response of armigeres subalbatus against bacteria.
Armigeres subalbatus is a natural vector of the filarial worm brugia pahangi, but it kills brugia malayi microfilariae by melanotic encapsulation. Download fulltext pdf download fulltext pdf mosquito transcriptome changes and filarial worm resistance in armigeres subalbatus article pdf available in bmc genomics 81. The cell lines, designated as ar3 and ar, consist of. In order to mine the transcriptome for new insight into the cascade of events that takes place in response to infection in this mosquito, 6 cdna libraries were generated from tissues of adult female. All structured data from the file and property namespaces is available under the creative commons cc0 license.
Pdf armigeres subalbatus incriminated as a vector of zoonotic. Laser beam points out the freeflight path of the insect in an increment of 41 ms. Armigeres subalbatus coquillett was the most susceptible species, indicating its status as a potential vector. To evaluate the mosquito larvicidal activity of plant extracts. Susceptibility of various mosquitoes of thailand to nocturnal. Armigeres subalbatus larvae are voracious predators of other mosquito larvae. Background to investigate mosquito larval habitats and resistance to common insecticides in areas with high incidence rates of mosquitoborne diseases in jining, shandong province, and to provide a scientific basis for the future prevention and control of mosquitoborne diseases and the rational use of insecticides. Culicidae across a temperate altitudinal gradient volume 105 issue 5 l. Immunolocalization of prophenoloxidase in the process of. Bulletin of the zoological survey, volume 4, issue 1. First molecular detection of plasmodium relictum in.
For example, in armigeres subalbatus po i is involved in melanization of microfilariae and po ii in hardening of egg chorion 7. Molecular detection of rickettsia felis and rickettsia. In an attempt to characterize all the pgrps in the mosquito armigeres subalbatus, two pgrp. This page was last edited on 15 september 2017, at 02.
Summary a twelve month indoor resting mosquito survey was carried out in five residential areas in kandy during 198586. In 2011, we reported occurrence of natural human infections with brugia pahangi, a filarial worm of dogs and cats, in a surburb of kuala lumpur, the capital city of malaysia. Jul 30, 20 in 2011, we reported occurrence of natural human infections with brugia pahangi, a filarial worm of dogs and cats, in a surburb of kuala lumpur, the capital city of malaysia. Effect of dichlorvos on spermatogenesis of armigeres. The mosquito, armigeres subalbatus, mounts a distinctively robust innate immune response when infected with the nematode brugia malayi, a causative agent of lymphatic filariasis. At present, 856 million people in 52 countries are living in areas that require preventive chemotherapy to stop the spread of this infection 1. Infections with wolbachia cause declines of host populations, and also induce host resistance to a wide range of pathogens.
Recently, two or more forms of propo cdnas have been isolated from mosquitoes, six forms are present in anopheles gambiae giles, one in anopheles stephensi liston, and two in aedes aegypti l. View enhanced pdf access article on wiley online library html view download pdf for offline viewing. Armigeres subalbatus incriminated as a vector of zoonotic brugia. Armigeres subalbatus coquillett is a medically important mosquito and a model species for immunology research. Dec 20, 2001 effect of prophenoloxidase expression knockout on the melanization of microfilariae in the mosquito armigeres subalbatus. Armigeres subalbatus was strongly associated with latrines with damaged or improperly sealed septic tank covers or 5. Armigeres subalbatus incriminated as a vector of zoonotic brugia pahangi filariasis in suburban kuala lumpur, peninsular malaysia. Armigeres armigeres subalbatus is a species complex of zoophilic mosquito belonging to the. Pdf the predatory nature of armigeres subalbatus larvae was examined to determine the possible use of this. Establishment and characterization of two new cell lines from the mosquito armigeres subalbatus coquillett diptera. The hemocyte libraries were not normalized, so this yield is not surprising. Armigeres subalbatus, brugia malayi, lymphatic filariasis, musi rawas, south sumatera. Mosquito oviposition in bamboo traps, with special reference. Our preliminary entomological survey at that time suggested the.
The mosquito armigeres subalbatus coquillet is a common vector of filariasis. Description of the transcriptomes of immune response. Rapid identification of medically important mosquitoes by matrix. Density fluctuation patterns o f cx quinquefasciatus and ae aegypti, were different among the five sites, probably due to local factors. A double subgenomic sindbis virus was used to silence ddc and assess its role in melanization of mf. Dec 01, 2008 armigeres subalbatus and several species of anopheles have shown strong melanization responses against invading nematodes hu 1941, christensen 1986, bangs et al. The biting cycle of armigeres subalbatus is distinctly crepuscular, exhibiting two peaks of activity, a smaller one at dawn. Show full abstract rate of cannibalism of 1st to 4th instar larvae of armigeres subalbatus were reported at 24 h intervals with and without alternative food supplement.